FIG. 7.

Expression profiles of the genes involved in the pathway for l-lysine biosynthesis in cells overexpressing DhGZF3. (A) The l-α-aminoadipate pathway for Lys biosynthesis in S. cerevisiae. (B) The ratio (log₂ base) of expression between control cells and cells overexpressing DhGZF3 for genes involved in Lys biosynthesis in synthetic medium (ammonium as nitrogen source) (filled bar) or rich medium (YPD) (empty bars) is represented. See the text for details. (C) Yeast cells bearing the empty plasmid pYPGE15 (−) or expressing DhGZF3 from pDhGZF3 (+) were grown in synthetic medium with Pro as the nitrogen source, and total RNA was prepared. RT-PCR was performed as described in Materials and Methods, and the product was visualized by agarose gel electrophoresis and ethidium bromide staining. M indicates 1-kbp ladder size markers.