Phenotypes associated with the gzf3, ure2, gln3, and gat1 mutations in S. cerevisiae. (A) Dilutions of wild-type strain BY4741 (+) and its isogenic gzf3 derivative were spotted onto plates containing the indicated compounds. Growth was monitored after 2 days. (B) Strains JK9-3da (WT), AS51-1a (ure2), TB105-3b (gln3 gat1), and AL11-2c (gln3 gat1 ure2) were spotted onto the indicated plates, and growth was monitored after 3 days. CFW, calcofluor white.