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The phosphorylation state of eRF1 does not affect NMD. Northern blot analysis of the CYH2 transcript was carried out on total RNA from the following strains: wild type (WT), upf1Δ, eRF1 S421A/S432A, and eRF1 S421D/S432D. For Northern blots, 25 μg of total RNA from the indicated strains was loaded into each lane.
