FIG. 1.

Construction of methionine-conditional C. albicans FBA1 mutants. One FBA1 allele was disrupted by insertional inactivation with the loxP-HIS1-loxP cassette, and the other FBA1 allele was placed under the control of the MET3 promoter. (A) Structure of the wild-type (FBA1), null (fba1::HIS1), and conditional (MET3-FBA1) alleles, with highlighting of the 5′ EcoRI (E)-HindIII (H) fragments detected by Southern blotting. (B) Southern blot of EcoRI-HindIII double-digested genomic DNA from the C. albicans FBA1 wild type, FBA1/FBA1 (RIM1000); the FBA1/fba1 (ARC101) and MET3-FBA1/FBA1 (ARC102) mutants; and three independent conditional MET3-FBA1/fba1 mutants (ARC103 to ARC105). The restriction fragments corresponding to each allele are indicated.