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The conditional C. albicans FBA1 mutants display a methionine-dependent growth defect on plates. C. albicans strains were grown at 30°C for 24 h on SC agar containing (+) or lacking (−) methionine (Met) and cysteine (Cys). Results for FBA1/FBA1 (RIM1000), FBA1/fba1 (ARC101), and MET3-FBA1/fba1 (ARC103) are shown. Strain ARC102 (MET3-FBA1/FBA1) grew similarly to RIM1000 and ARC101, whereas ARC104 and ARC105 displayed phenotypes similar to that of ARC103 (not shown).
