FIG. 5.

Relationship between level of active Fba1p and growth of C. albicans. C. albicans RM1000 (wild type [WT]) and ARC103 (MET3-FBA1/fba1) were grown in SC medium containing methionine and cysteine at 30°C, and their growth was monitored (optical density at 600 nm). The levels of active Fba1p were measured at various times during exponential growth, and Fba1p levels in the mutant were expressed as percentages of those in wild-type cells. The doubling times of the cultures were monitored at the time of Fba1p measurement, and those of mutant cells were expressed as percentages of those of wild-type cells. Results for experiment 1 (black squares) and experiment 2 (black circles) are shown. These doubling times reflect the rate of generation of new biomass and hence the biochemical flux to “growth.”