TABLE 2.

PCR primers

Primer	Use	Sequence (5′-3′)
MUF-F	Amplification of URA3-MET3-FBA1 cassette	AGTGAGTGAGTGAGTGGTGTGTCATAATAATGAATTGTCTGTTGGTGGTGGTGGTTTGATTAGCACCACCACCATACATAACTAATAGGAATTGATTGGATGG
MUF-R	Amplification of URA3-MET3-FBA1 cassette	TCTTGAGCATAGTCAAACAAGTCTTTGACGTCTTTACCGTAGATAACACCGGATTTACTTAAAACTGCTGGAGGAGCCATGGGGAGGGTATTTACTTTTAAATA
LHL-F	Amplification of fba1::HIS1 cassette	CCCAATCTTCCCGTTCTTTCAAATCAAAAATTTTTCAACCCCCTTTTCTTTATTTCTTTTCTTTTGTTTTTGTTCTACACCCTCTTCGCTATTACGCCAG
LHL-R	Amplification of fba1::HIS1 cassette	CAACACTGCTATCAAGAATGGTGTTGTCAAGGTCAACTTGGACACTGATTGTCAATATGCTTACTTGACTGGTATCAGAGGCAGATTACCCTGTTATCCCTA
FBA-F3	Diagnosis of FBA1 and fba1::HIS1 alleles	CAGGCAGAAAGTGGGAGC
FBA-R3	Diagnosis of FBA1 and URA3-MET3-FBA1 alleles	CGGCTTTCAACATACCATCA
MET3p-F	Diagnosis of URA3-MET3-FBA1 allele	ATTGCTGTGGATCACGTGC
HIS-R1	Diagnosis of fba1::HIS1 allele	CACAAGAAGCCTCAACGG
FBA-F4	Amplification of FBA1 probe for Southern analysis	AGGATCAGTTTTTCCCCGG
FBA-R4	Amplification of FBA1 probe for Southern analysis	GCTAATCAAACCACCACCACC