TABLE 1.
Primer pairs designed to amplify each of 37 stevor genes and 3 var genes
| Primer set | Primer sequence
|
Target gene(s) | |
|---|---|---|---|
| Forward | Reverse | ||
| 1 | TGATGCCCTTGCTAGTTATGC | CAGAAGTTGCAGCAGGTACTGT | PFA0090c |
| 3 | CAGCTATTCAAGCAGGTGCTAA | CAATACCACAACCTCCAGGAA | PFA0705c |
| 5 | GGTTTGGCAAAGGCAAAATA | CAAGCTGAACTACCAGCTTCAA | PFA0105w |
| 7 | GGCTGCCTTTGATACCTTGA | TGTACCGCCTGCAAACATAG | PFA0750w |
| 9 | GCAAAATCTGCTGCCCTTAC | ACCATTTGCACAGGTTCCAC | PFB0065w |
| 11 | AAGGCGCTTGCTGGTATCTA | CTCGCAATACTAGCCATTCTCA | PFB0995w |
| 15 | TGCTGCTGTCACTTCTAGCTTT | CTACACCTCCTGCTGCAGTAAC | PFB1020w |
| 18 | AAAGGTGCTGCTATTTCTACCG | CAGCAGCACAACCTACTGTACC | PFC0025c |
| 20 | TGCATGCTGCTAAAGTTGCT | TTGGTTGCACAAAGAACTGACT | PFC1105w |
| 22 | AAATGCGCATCCTCTATCACT | AGTTCCGACCCCATCACTAA | PFD0065w |
| 24 | TTTATGGTATTAGCGCTGCAAG | GCGCTTTCTATTGATGTTTGAG | PFD0125c |
| 26 | TAGTTGTGTGTGCCCTTTCG | TTCGTCAATTGGGATGATGA | PFD0220c |
| 28 | TGGCAGCTACCAAAGCTACA | TCAGGACCCCAAGCTGTAATA | PFD0035c |
| 30 | GCAAAGGTTCTTGCTGGTGT | GCTATTCTCATAGCATCCCAATG | PFE0030c |
| 34 | GTTGATGCCATCCTTCCTGT | ACGCTTCGGTTGCTTTAAGA | PFF1550w |
| 36 | GGCTGCACTTGCTTACTTTTC | TAGCACATGTTGCACCTCCT | PFI0080w |
| 41 | TGGGGCTCCTACATTAAGTCA | CACGACAATTGCCATACCAT | PF10_0009 |
| 43 | AGGTGCTCTTGCTGAGTATGC | CAAATTTCAGTGCCTGCTTG | PF10_0395 |
| 45 | TGACGTTGCTGCCTTGAATA | ACCAACGCCTGCTTGAATAG | PF11_0516 |
| 47 | AGAACGTTGTGTTGGAGGTGT | GGAAGCCATAAACCAACAGG | PF11_0013 |
| 51 | GCCGTTGCTTCTCTTGTTTATT | ATCAGTAGCACCGGCAAGAG | PF13_0009 |
| 53 | GCAAAAGTTGCTGTCATTGG | GAGCCTGCTGCATTAACTGA | PF14_0767 |
| 55 | CATGAAGGCTGTTGCTGATTAT | ATCAGTAGCACCCTCAACACAT | PF14_0771 |
| 57 | TACGTGCGCATCCCATATTA | TTCCGACTAAATCACCAGCA | MAL7P1.218 |
| 59 | GCAAAAGCTGCTATCCTTGG | ACACCACCTGATGCACTAACTG | MAL8P1.217 |
| 61 | CTGCTAAAACGTGTGCGTCT | ATTTGCAGCAGGCAAAACTA | MAL13P1.505 |
| 63 | AAGTGAGATGTTCCCGTGGT | TGGACAGTAAAGTGCGCAAG | MAL7P1.223 |
| 65 | AATTGTCCCAAACCCTTGTC | AGCCTTCGTAGCCATTGAAC | MAL7P1.310 |
| 67 | TTGCAGAAGTGCCTAAGAATTG | GCACTAACAGTAGTTGCTTCTCCT | PF07_0130 |
| 69 | TGCGTGCAAATCCTCTATCA | AATTGCACCAGCAGAAGTTG | PFL2610w |
| 71 | CTGCTGCCATTGCTACCTTT | AGCAGCACCAGATGCACATA | PFB0050c |
| 73 | CTTCCGTTGGACCACCTTAT | CTTGCAGCAGTTCCGGTTAT | PF14_0007 |
| 77 | GCTAAAACGGCTGCCCTAA | CACCACTTGCACAAAATCCA | PFL2620w/PFL2635w |
| 79 | CTGCACCTTATGATGCTTGTGT | AAACAGTTCCGGCCATATCA | PFF0850c |
| 80 | TTGCAGAAGTGCCTAAGAATTG | CATAGCAGCACTTGCAGCAG | PFI0045c |
| 83 | GCCCTAAGTGCTGTTGCTTC | CACCAACAACACATGCTTTCA | PFB0025c |
| A | TTGTCCGCAAAACGATACAA | TGGAGGGACTTTTTCGTCTG | PFD0625c (CIDR2 non-α) |
| B | GTAGCGTCCTCGGTGAAGAG | TGCTGTTACCCACCAAAATG | PFD1015c (DBL2δ) |
| C | GAGCTGCGAGGTCAGAAAAT | AAGGGGTATCACACGCAGTT | PF07_0051 (CIDR2 non-α) |