TABLE 1.

Increases in hpdA mRNA levels in response to different carbon sources

Treatmenta	Fold increase in mRNA expression ± SDb	Relative fold increase in transcript levelc
0 h	35.0 ± 6.12	1
Glucose, 2 h	0.03 ± 0.002	0.0009
Glucose, 4 h	0.04 ± 0.002	0.0010
Glucose, 6 h	0.05 ± 0.007	0.0013
Glucose, 8 h	7.88 ± 3.14	0.2254
Phe, 2 h	1,553.10 ± 104.85	44.42
Phe, 4 h	1,985.32 ± 104.35	56.79
Phe, 6 h	2,408.05 ± 147.0	68.88
Phe, 8 h	1,559.27 ± 96.0	44.60
Phe+Glu, 2 h	1.82 ± 0.67	0.052
Phe+Glu, 4 h	0.27 ± 0.02	0.008
Phe+Glu, 6 h	2.34 ± 0.26	0.067
Phe+Glu, 8 h	9.26 ± 0.10	0.265
Phe+Lac, 2 h	630.88 ± 104.5	18.04
Phe+Lac, 4 h	1,089.40 ± 18.39	31.16
Phe+Lac, 6 h	1,095.63 ± 3.61	31.34
Phe+Lac, 8 h	2,096.03 ± 44.0	59.95

^aMycelia were grown in MM for 16 h at 37°C and then transferred to either MM (glucose), MC+Phe, MC+Phe+Glu, or MC+Phe+Lac and grown for 2, 4, 6, or 8 h at 37°C. Real-time RT-PCR was the method used to quantify the mRNA. The measured quantity of the hpdA mRNA in each of the treated samples was normalized using the cycle threshold values obtained for the tubC RNA amplifications run in the same plate. The relative quantitation of all the genes and tubulin gene expression was determined by a standard curve (i.e., cycle threshold, values plotted against logarithm of the DNA copy number).

^bNumber of copies of cDNA divided by the number of copies of β-tubulin cDNA. The results are the averages of four sets of experiments. The results were expressed as means ± standard deviations.

^cThe values represent the number of times the genes are expressed compared to the control (0 h) grown for 16 h in MM (represented absolutely as 1.00).