TABLE 1.
Treatmenta | Fold increase in mRNA expression ± SDb | Relative fold increase in transcript levelc |
---|---|---|
0 h | 35.0 ± 6.12 | 1 |
Glucose, 2 h | 0.03 ± 0.002 | 0.0009 |
Glucose, 4 h | 0.04 ± 0.002 | 0.0010 |
Glucose, 6 h | 0.05 ± 0.007 | 0.0013 |
Glucose, 8 h | 7.88 ± 3.14 | 0.2254 |
Phe, 2 h | 1,553.10 ± 104.85 | 44.42 |
Phe, 4 h | 1,985.32 ± 104.35 | 56.79 |
Phe, 6 h | 2,408.05 ± 147.0 | 68.88 |
Phe, 8 h | 1,559.27 ± 96.0 | 44.60 |
Phe+Glu, 2 h | 1.82 ± 0.67 | 0.052 |
Phe+Glu, 4 h | 0.27 ± 0.02 | 0.008 |
Phe+Glu, 6 h | 2.34 ± 0.26 | 0.067 |
Phe+Glu, 8 h | 9.26 ± 0.10 | 0.265 |
Phe+Lac, 2 h | 630.88 ± 104.5 | 18.04 |
Phe+Lac, 4 h | 1,089.40 ± 18.39 | 31.16 |
Phe+Lac, 6 h | 1,095.63 ± 3.61 | 31.34 |
Phe+Lac, 8 h | 2,096.03 ± 44.0 | 59.95 |
Mycelia were grown in MM for 16 h at 37°C and then transferred to either MM (glucose), MC+Phe, MC+Phe+Glu, or MC+Phe+Lac and grown for 2, 4, 6, or 8 h at 37°C. Real-time RT-PCR was the method used to quantify the mRNA. The measured quantity of the hpdA mRNA in each of the treated samples was normalized using the cycle threshold values obtained for the tubC RNA amplifications run in the same plate. The relative quantitation of all the genes and tubulin gene expression was determined by a standard curve (i.e., cycle threshold, values plotted against logarithm of the DNA copy number).
Number of copies of cDNA divided by the number of copies of β-tubulin cDNA. The results are the averages of four sets of experiments. The results were expressed as means ± standard deviations.
The values represent the number of times the genes are expressed compared to the control (0 h) grown for 16 h in MM (represented absolutely as 1.00).