TABLE 3.

Mapping of SAGE tags to the 221ES^a

Tag ID no.b	Tag sequence	Annotation	Frequency in:		R value
			427-221 library	427-800 library
41524	CATGTGGTATGTACAAGCTAC	esag6/7c	44	25	1.13
41878	CATGGGGAACACTGCTGTGCT	esag6	32	32	0.00
43692	CATGTAAGGAAACGGCAACAG	Downstream of esag6	58	45	0.34
63	CATGATATAAAACGTGGATGC	esag5Ψ	40	45	0.07
87	CATGAAAATCCTGGGGCAGCA	esag3/3Ψd	34	28	0.12
42811	CATGTGGGGAGGGGTGACTTT	esag4	46	38	0.16
42492	CATGATCTTTTTTTTCTGATT	Hypothetical protein H25N7.21	38	39	0.00
501	CATGGAATTGATGTCTCTTCC	esag8/8be	10	2	1.25
41933	CATGTGCCTTGTGCTTCTTTT	Hypothetical protein H25N7.25	15	2	2.43
263	CATGTGCACTGGACTATGAGC	esag8b	15	0	4.50
177	CATGAAAATCCTGAGGCAGAA	esag3	20	0	6.00
56	CATGTGAGGGAGAACTAAAAA	esag1	44	0	13.16

^aTags are approximately sorted by position on the 221ES (GenBank accession no. AL671259) (Fig. 8). Data presented in bold represent tags with sequences entirely unique to the 221ES. All other tag sequences can be mapped to other known T. b. brucei expression sites but not non-ES locations.

^bID, identification.

^cTag no. 41524 cannot discriminate between esag6 and esag7 transcripts.

^dTag no. 87 maps to both the esag3 pseudogene and the functional esag3 copy in the middle of the ES. It does not map to the terminal copy of esag3.

^eTag no. 501 maps to both copies of esag8 as well as to esag8b. As tag no. 263 independently suggests an absence of esag8b transcripts in 427-800, we assume esag8b does not contribute to the frequency of tag no. 501 in 427-800.