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. 2006 Aug;5(8):1420–1429. doi: 10.1128/EC.00078-06

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Copyright © 2006, American Society for Microbiology

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FIG. 7. — PI-PLC treatment of permeabilized T. gondii tachyzoites. Hypotonically permeabilized tachyzoites were labeled in vitro via UDP-[6-³H]GlcNAc in the presence of EDTA and absence of Mn²⁺ (A to C), GDP-[2-³H]Man (D to F), or UDP-[6-³H]Glc (G to I). Glycolipids were extracted and analyzed by TLC before (A, D, and G) and after B. thuringiensis PI-PLC treatment in the absence (B, E, and H) or presence (C, F, and I) of Triton X-100. Radioactivity was detected by a Berthold TLC scanner (LB 1842).