Tests for FLC MIC for the ancestor (diamonds), strains carrying PDR1r and UNK1r from generation 400 of evolution experiment 1 (squares), the mutant strain carrying erg3r (circles), and offspring strains carrying both PDR1r and erg3r (triangles), among which segregation of UNK1r and UNK1s is expected to occur. In addition, strains carrying the respective PDR1r mutation at generation 100 (stars) are included for comparison.