FIG. 3.

Uptake and efflux of rhodamine 6G. The protocol used followed that of Kolaczkowski et al. (13); see Materials and Methods for details. The results are from one of two replicate experiments and are representative of the two. RHO was added at time zero. Note that the erg3^r and PDR1^r/erg3^r strains take up RHO faster initially than any of the other strains, before all converge to the same level of uptake. Glucose-mediated efflux occurred in all strains except the PDR5 deletion strain. Cells of all strains maintained viability throughout the experiment.