TABLE 1.
Amplification primers and hybridization probes
| Primer or probe | Description of mutationa | Sequence |
|---|---|---|
| Amplification primers | ||
| PDR1 (5′ end) | TGGAAACAGACGCATACCC | |
| CACTGAGAATGCTTGTGATACC | ||
| PDR1 (3′ end) | GACAACATGTGCATATCCGAC | |
| ACCTGTTGAAACCAAGAGCC | ||
| PDR3 | ACGAGGTTCTCCTTTTGGACA | |
| AGACCGAACAGAAAGGACAGA | ||
| ERG3 | TTTGAAGTGGTTGCAGAGGA | |
| AGACAAACAAGGCAACCGTA | ||
| Allele-specific hybridization probes | ||
| PDR1 | Wild type | TTCCTGACAATCGTC |
| T817K | TTCCTGAAAATCGTC | |
| PDR1 | Wild type | CTCTTGCGCTTATC |
| C862W | CTCTTGGGCTTATC | |
| PDR1 | Wild type | ATATACCTTCACAAG |
| L722P | ATATACCCTCACAAG | |
| PDR3 | Wild type | AGGTATAGGATATTTA |
| G253V | AGGTATAGTATATTTA | |
| PDR1 | Wild type | AAATTATAGATCCAAAT |
| R289I | AAATTATATATCCAAAT | |
| PDR3 | Wild type | GGGCATGTTTGGAC |
| C707F | GGGCATTTTTGGAC | |
| PDR3 | Wild type | GGAGTTTGTCAGCA |
| V954F | GGAGTTTTTCAGCA | |
| PDR3 | Wild type | CGTGAGTTTTTTGAT |
| F815Y | CGTGAGTATTTTGAT | |
| ERG3 | Wild type | CCCATGGATGTCG |
| A148* | CCCATGAATGTCG |
a
*, nonsense mutation.