TABLE 1.
Primer or probe | Description of mutationa | Sequence |
---|---|---|
Amplification primers | ||
PDR1 (5′ end) | TGGAAACAGACGCATACCC | |
CACTGAGAATGCTTGTGATACC | ||
PDR1 (3′ end) | GACAACATGTGCATATCCGAC | |
ACCTGTTGAAACCAAGAGCC | ||
PDR3 | ACGAGGTTCTCCTTTTGGACA | |
AGACCGAACAGAAAGGACAGA | ||
ERG3 | TTTGAAGTGGTTGCAGAGGA | |
AGACAAACAAGGCAACCGTA | ||
Allele-specific hybridization probes | ||
PDR1 | Wild type | TTCCTGACAATCGTC |
T817K | TTCCTGAAAATCGTC | |
PDR1 | Wild type | CTCTTGCGCTTATC |
C862W | CTCTTGGGCTTATC | |
PDR1 | Wild type | ATATACCTTCACAAG |
L722P | ATATACCCTCACAAG | |
PDR3 | Wild type | AGGTATAGGATATTTA |
G253V | AGGTATAGTATATTTA | |
PDR1 | Wild type | AAATTATAGATCCAAAT |
R289I | AAATTATATATCCAAAT | |
PDR3 | Wild type | GGGCATGTTTGGAC |
C707F | GGGCATTTTTGGAC | |
PDR3 | Wild type | GGAGTTTGTCAGCA |
V954F | GGAGTTTTTCAGCA | |
PDR3 | Wild type | CGTGAGTTTTTTGAT |
F815Y | CGTGAGTATTTTGAT | |
ERG3 | Wild type | CCCATGGATGTCG |
A148* | CCCATGAATGTCG |
*, nonsense mutation.