FIG. 2.

Gcn5 is required for growth under specific environmental stress conditions. The gcn5⁻ mutant spotted on YEA plates at 36°C or YEA plates supplemented with 1 M KCl or 100 mM CaCl₂ displayed reduced growth relative to the wild type (wt), whereas gcn5⁻ mutant cells spotted on YEA plates supplemented with 1.2 M sorbitol showed no difference in growth from wild-type cells. Plates were incubated for 3 to 5 days at 25°C unless otherwise indicated.