FIG. 4.

Rvs167-GFP does not localize in a polarized manner in the myo5Δ and sla2Δ mutants. (A) Partial colocalization of Rvs167p-GFP with cortical actin patches in wild-type hyphal tips. Hyphal cells expressing Rvs167p-GFP were fixed, stained with rhodamine-phalloidin (RP), and visualized by epifluorescence microscopy. (B) Wild-type and mutant hyphal forms expressing Rvs167p-GFP were visualized by epifluorescence microscopy. Arrowheads point to mutant hyphal tips showing Rvs167-GFP signal. Bar = 10 μm.