FIG. 8.

(A) Hog1p is not phosphorylated in the myo5Δ and sla2Δ mutants grown in YPD but becomes phosphorylated when cells are treated with NaCl. Phospho-Hog1p was detected by Western blot analysis in wild-type and mutant strains grown in YPD (−) and stressed with 0.5 M NaCl for 3 min (+), using anti-phospho p38 antibodies. Hog1p protein levels were also detected using anti-p38 antibodies. (B) The hog1Δ and myo5Δ strains exhibit different salt sensitivities. Tenfold dilutions of overnight cultures of wild-type, hog1Δ, myo5Δ, and hog1 myo5Δ strains were spotted on solid medium containing various salts.