FIG. 3.

Phenotypes of Δgpr-4 mutants. (A) Dry mass using 100 mM glucose, glycerol, mannitol, or arabinose as a sole carbon source in solid medium. One-microliter aliquots containing 1 × 10⁹ conidia were inoculated in the center of cellophane-overlaid plates. Tissues were collected after incubation for 3 days at 30°C in the dark, and dry mass was measured as described in Materials and Methods. Strains were 74A (wild type) and 7-32 (Δgpr-4 mutant). Data are from three independent experiments, with three replicates/experiment. Errors are calculated as standard errors. (B) Δgpr-4 mutants have fewer hyphae than the wild type when cultured on glycerol medium. VM-glycerol flasks were inoculated in the center using 1 × 10⁹ conidia (in 1 μl) and then incubated for 3 days in the dark at 30°C, followed by four more days under light at 25°C. Strains are the same as for Fig. 2D. (C) Cell density-dependent mass formation on glycerol solid medium. The indicated amounts of conidia were spread evenly onto the surface of 35-mm-diameter VM-glycerol plates, followed by incubation in the dark at 30°C. Plates were photographed after 8 days. Strains are 74A (wild type) and 7-32 (Δgpr-4 mutant).