TABLE 1.
N. crassa strains
| Strain | Relevant genotype | Comment(s) | Source or reference |
|---|---|---|---|
| 74A-OR23-1A (74A) | Wild-type matA | FGSCa 987 | FGSC |
| 74a-OR8-1a (74a) | Wild-type mata | FGSC 988 | FGSC |
| 7-32 | Δgpr-4::hph+matA | Δgpr-4 homokaryon | This study |
| 7-33 | Δgpr-4::hph+mata | Δgpr-4 homokaryon | This study |
| 35-6 | Δgpr-4::hph+ mata | Δgpr-4 homokaryon | This study |
| 38-2 | Δgpr-4::hph+ mata | Δgpr-4 homokaryon | This study |
| LA25 | pan-2 matA | pan-2 mutant | R. L. Weiss, UCLA |
| Rmi2 | Δgpr-4::hph+gpr-4+::his-3+mata | Complemented Δgpr-4 | This study |
| FGSC 6103 | his-3 matA | his-3 targeting strain | FGSC |
| FGSC 4008 | cr-1 matA | Allele B123 | FGSC |
| 7-33his3A | Δgpr-4::hph+his-3 matA | FGSC 6103 × 7-33 progeny | This study |
| 35-6pan2-7 | Δgpr-4::hph+ pan-2 mata | 35-6 × LA25 progeny | This study |
| 35-6pan2-8 | Δgpr-4::hph+ pan-2 matA | 35-6 × LA25 progeny | This study |
| 1B8 | Δgna-1::hph+ matA | gna-1 mutant | 29 |
| a1r54 | Δgna-1::hph+ Δgpr-4::hph+matA | 1B8 × 7-33 progeny | This study |
| a1r2 | Δgna-1::hph+ Δgpr-4::hph+mata | 1B8 × 7-33 progeny | This study |
| a1r2h7 | Δgna-1::hph+ Δgpr-4::hph+his-3 | FGSC 6103 × a1r2 progeny | This study |
| a29-1 | Δgna-2::pyrG+matA | gna-2 mutant | 3 |
| a2r2 | Δgna-2::pyrG+ Δgpr-4::hph+matA | A29-1 × 7-33 progeny | This study |
| 31c2 | Δgna-3::hph+matA | gna-3 mutant | 33 |
| 2a1 | Δgna-3::hph+ matA | gna-3 mutant | 33 |
| a3r1 | Δgna-3::hph+ Δgpr-4::hph+matA | 2a1 × 7-33 progeny | This study |
| Δ1gna-1Q204L | Δgna-1::hph+ gna-1Q204L::his-3+matA | gna-1Q204L allele | H. Kim and K. A. Borkovich, unpublished data |
| a1rQ204L-25 | Δgpr-4::hph+ Δgna-1::hph+gna-1Q204L::his-3+ mata | Δgpr-4 gna-1Q204L allele | This study |
| a1rR178C | Δgpr-4::hph+ Δgna-1::hph+ gna-1R178C::his-3+ mata | Δgpr-4 gna-1R178C allele | This study |
a
FGSC, Fungal Genetics Stock Center, Kansas City, Mo.