TABLE 2.
Properties of H. influenzae isolates used in this study
| Serotype-isolate namea | Geographic origin | Date of isolation | Allelic profile
|
ST | MLEE lineageb | ETb | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| adk | atpG | frdB | fucK | mdh | pgi | recA | ||||||
| BPF-F3028 | Brazil | 1986 | 24 | 26 | 24 | 21 | 36 | 33 | 21 | 65 | ||
| b-RM7017 | Ghana | 1980s | 10 | 14 | 5 | 7 | 26 | 22 | 8 | 31 | A1e | 52 |
| b-RM7419 | Kenya | 1980s | 10 | 14 | 5 | 7 | 4 | 7 | 8 | 53 | A2a | 60 |
| b-RM7118 | Australia | 1985 | 9 | 14 | 12 | 3 | 4 | 3 | 8 | 22 | A1a | 1.7 |
| b-RM7717 | Australia | 1989 | 9 | 14 | 12 | 3 | 4 | 3 | 8 | 22 | ||
| b-Eagan | United States | 1950s | 10 | 14 | 4 | 3 | 4 | 3 | 8 | 44 | A1a | 6 |
| b-RM6107 | England | 1985 | 10 | 14 | 4 | 3 | 4 | 3 | 8 | 44 | A1a | 31 |
| b-RM7020 | Ghana | 1983 | 10 | 14 | 4 | 3 | 4 | 3 | 8 | 44 | A1d | 50 |
| b-RM7430 | Kenya | 1980s | 10 | 14 | 4 | 3 | 4 | 3 | 8 | 44 | A1a | 8 |
| b-M6587 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 3 | 4 | 3 | 8 | 44 | ||
| b-M6594 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 3 | 4 | 3 | 8 | 44 | ||
| b-M6603 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 3 | 4 | 3 | 8 | 44 | ||
| b-M6604 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 3 | 4 | 3 | 8 | 44 | ||
| b-M6605 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 3 | 4 | 3 | 8 | 44 | ||
| b-M6606 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 3 | 4 | 3 | 8 | 44 | ||
| b-M6607 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 3 | 4 | 3 | 8 | 44 | ||
| b-RM8012 | United States | 1984 | 10 | 14 | 4 | 5 | 4 | 31 | 8 | 55 | A2e | 122 |
| b-RM6094 | England | 1983 | 23 | 20 | 21 | 5 | 4 | 7 | 8 | 64 | A2a | 71 |
| b(iv)-7853 | United States | Before 1991 | 10 | 14 | 22 | 5 | 4 | 7 | 22 | 54 | ||
| b(iv)-7854 | United States | Before 1991 | 10 | 14 | 22 | 5 | 4 | 7 | 22 | 54 | ||
| b(iv)-7863 | United States | Before 1991 | 10 | 14 | 22 | 5 | 4 | 7 | 22 | 54 | ||
| b(iv)-7868 | United States | Before 1991 | 10 | 14 | 22 | 5 | 4 | 7 | 22 | 54 | ||
| b(iv)-7871 | United States | Before 1991 | 10 | 14 | 22 | 5 | 4 | 7 | 22 | 54 | ||
| b(iv)-7884 | United States | Before 1991 | 10 | 14 | 22 | 5 | 4 | 7 | 22 | 54 | ||
| b(iv)-7885 | United States | Before 1991 | 10 | 14 | 22 | 5 | 4 | 7 | 22 | 54 | ||
| b(iv)-7887 | United States | Before 1991 | 10 | 14 | 22 | 5 | 4 | 7 | 22 | 54 | ||
| b(iv)-7894 | United States | Before 1991 | 10 | 14 | 22 | 5 | 4 | 7 | 22 | 54 | ||
| b(iv)-7909 | United States | Before 1991 | 10 | 14 | 22 | 5 | 4 | 7 | 22 | 54 | ||
| b(iv)-7910 | United States | Before 1991 | 10 | 14 | 22 | 5 | 4 | 7 | 22 | 54 | ||
| b-M6596 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6597 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6598 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6599 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6600 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6612 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6613 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6614 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6615 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6616 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6617 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6618 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6619 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6624 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-M6894 | Pennsylvania | 1999-2000 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-RM7651 | Norway | 1976 | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | ||
| b-RM7109 | Sweden | 1985 | 10 | 14 | 4 | 5 | 4 | 7 | 20 | 24 | A2a | 21.9 |
| b-RM7414 | Kenya | 1980s | 6 | 20 | 23 | 1 | 33 | 29 | 23 | 50 | B1b | 140 |
| b-M6588 | Pennsylvania | 1999-2000 | 6 | 20 | 20 | 1 | 33 | 29 | 7 | 45 | ||
| b-M6589 | Pennsylvania | 1999-2000 | 6 | 20 | 20 | 1 | 33 | 29 | 7 | 45 | ||
| b-M6755 | Pennsylvania | 1999-2000 | 6 | 20 | 20 | 1 | 33 | 29 | 7 | 45 | ||
| d-RM7271 | Malaysia | 1972 | 5 | 15 | 10 | 15 | 8 | 5 | 11 | 48 | B1g | 162 |
| d-RM7429 | Kenya | 1986 | 5 | 15 | 10 | 8 | 8 | 5 | 11 | 49 | B1g | 163 |
| d-RM6137 | England | 1963 | 5 | 15 | 10 | 9 | 8 | 5 | 11 | 10 | B1f | 157 |
| d-RM7033 | Papua New Guinea | 1980s | 5 | 15 | 10 | 9 | 8 | 5 | 11 | 10 | B1f | 161 |
| d-RM1168 | United States | 1983 | 5 | 15 | 7 | 9 | 7 | 5 | 11 | 47 | B1f | 158 |
| d-RM6150 | England | 1985 | 5 | 15 | 7 | 9 | 7 | 5 | 11 | 47 | B1f | 159 |
| d-RM8039 | United States | 1984 | 5 | 15 | 7 | 9 | 7 | 5 | 11 | 47 | B1f | 160 |
| d-Rd | United States | Before 1952 | 5 | 15 | 7 | 9 | 7 | 5 | 11 | 47 | ||
| nt-486 | Finland | 1994-1995 | 17 | 4 | 17 | 1 | 7 | 12 | 11 | 41 | ||
| a-RM1042 | United States | 1981 | 12 | 5 | 5 | 2 | 3 | 11 | 7 | 5 | B2a | 164 |
| a-RM7190 | Malaysia | 1973 | 13 | 16 | 5 | 2 | 3 | 11 | 7 | 23 | B2c | 169 |
| a-RM7191 | Malaysia | 1973 | 13 | 16 | 5 | 2 | 3 | 11 | 7 | 23 | B2b | 167 |
| a-RM7031 | Papua New Guinea | 1980s | 13 | 16 | 5 | 18 | 3 | 11 | 7 | 56 | B2b | 165 |
| a-RM7032 | Papua New Guinea | 1980s | 13 | 16 | 5 | 18 | 3 | 11 | 7 | 56 | B2b | 166 |
| a-RM7198 | Malaysia | 1974 | 13 | 16 | 5 | 18 | 3 | 11 | 7 | 56 | B2b | 168 |
| a-RM7205 | The Gambia | 1980s | 4 | 17 | 4 | 1 | 2 | 9 | 6 | 4 | B4 | 173 |
| a-RM7416 | Kenya | 1986 | 4 | 17 | 4 | 1 | 2 | 9 | 6 | 4 | B4 | 174/PICK> |
| nt-1008 | Finland | 1994-1995 | 1 | 22 | 19 | 19 | 32 | 17 | 2 | 43 | ||
| c-RM6134 | England | 1975 | 7 | 11 | 6 | 7 | 6 | 10 | 10 | 8 | D4 | 185 |
| c-RM7422 | Kenya | 1986 | 7 | 11 | 6 | 7 | 6 | 10 | 10 | 8 | D5 | 186 |
| c-RM6132 | England | 1964 | 7 | 11 | 6 | 6 | 18 | 16 | 9 | 19 | D2 | 183 |
| c-RM8032 | United States | 1983 | 14 | 20 | 6 | 8 | 6 | 16 | 21 | 58 | D3 | 184 |
| c-RM7267 | Malaysia | 1973 | 7 | 2 | 6 | 6 | 6 | 16 | 9 | 51 | D1b | 180 |
| c-RM7270 | Malaysia | 1975 | 7 | 11 | 6 | 6 | 6 | 16 | 9 | 7 | D1a | 178 |
| c-RM7424 | Kenya | 1986 | 7 | 11 | 6 | 6 | 6 | 16 | 9 | 7 | E1 | 187 |
| c-RM1167 | United States | Before 1983 | 7 | 11 | 6 | 8 | 6 | 16 | 9 | 9 | D1a | 177 |
| c-RM1271 | Probably United States | 1968 | 7 | 11 | 6 | 8 | 6 | 16 | 9 | 9 | D1b | 179 |
| nt-M6593 | Pennsylvania | 1999-2000 | 1 | 25 | 1 | 14 | 15 | 1 | 5 | 46 | ||
| nt-981 | Finland | 1994-1995 | 2 | 24 | 18 | 18 | 27 | 1 | 5 | 42 | ||
| nt-477 | Finland | 1994-1995 | 1 | 1 | 1 | 14 | 15 | 1 | 5 | 1 | ||
| nt-432 | Finland | 1994-1995 | 1 | 3 | 1 | 1 | 31 | 1 | 5 | 40 | ||
| nt-1124 | Finland | 1994-1995 | 1 | 1 | 1 | 13 | 13 | 25 | 16 | 12 | ||
| nt-1247 | Finland | 1994-1995 | 1 | 10 | 1 | 1 | 1 | 6 | 5 | 33 | ||
| nt-375 | Finland | 1994-1995 | 1 | 1 | 1 | 1 | 1 | 1 | 5 | 3 | ||
| nt-723 | Finland | 1994-1995 | 5 | 1 | 1 | 1 | 1 | 2 | 5 | 14 | ||
| nt-1180 | Finland | 1994-1995 | 14 | 7 | 1 | 15 | 16 | 4 | 1 | 2 | ||
| nt-1181 | Finland | 1994-1995 | 14 | 7 | 1 | 15 | 16 | 4 | 1 | 2 | ||
| nt-1292 | Finland | 1994-1995 | 14 | 7 | 1 | 15 | 16 | 4 | 1 | 2 | ||
| nt-1200 | Finland | 1994-1995 | 16 | 21 | 14 | 16 | 29 | 14 | 3 | 36 | ||
| nt-1268 | Finland | 1994-1995 | 16 | 21 | 14 | 16 | 29 | 14 | 3 | 36 | ||
| nt-285 | Finland | 1994-1995 | 15 | 23 | 16 | 16 | 30 | 1 | 3 | 39 | ||
| nt-1231 | Finland | 1994-1995 | 11 | 2 | 15 | 8 | 28 | 26 | 3 | 34 | ||
| nt-1232 | Finland | 1994-1995 | 11 | 2 | 15 | 8 | 28 | 26 | 3 | 34 | ||
| nt-176 | Finland | 1994-1995 | 10 | 2 | 15 | 17 | 26 | 26 | 3 | 38 | ||
| nt-1158 | Finland | 1994-1995 | 1 | 8 | 1 | 14 | 9 | 14 | 13 | 11 | ||
| nt-1159 | Finland | 1994-1995 | 1 | 8 | 1 | 14 | 9 | 14 | 13 | 11 | ||
| nt-1207 | Finland | 1994-1995 | 3 | 9 | 8 | 4 | 14 | 8 | 4 | 13 | ||
| nt-1209 | Finland | 1994-1995 | 3 | 9 | 8 | 4 | 14 | 8 | 4 | 13 | ||
| nt-1233 | Finland | 1994-1995 | 3 | 9 | 8 | 4 | 14 | 8 | 4 | 13 | ||
| nt-162 | Finland | 1994-1995 | 14 | 7 | 13 | 4 | 1 | 21 | 1 | 37 | ||
| nt-667 | Finland | 1994-1995 | 14 | 7 | 13 | 7 | 17 | 13 | 17 | 57 | ||
| a-RM6064 | England | 1966 | 20 | 12 | 1 | 7 | 20 | 23 | 19 | 21 | H1b | 223 |
| a-RM6068 | England | 1968 | 20 | 12 | 25 | 7 | 20 | 23 | 19 | 30 | H1b | 226 |
| a-RM6083 | England | 1977 | 20 | 12 | 21 | 20 | 34 | 30 | 19 | 60 | H1a | 220 |
| a-RM6070 | England | 1962 | 20 | 12 | 1 | 8 | 20 | 23 | 19 | 59 | H1b | 224 |
| a-RM6073 | England | 1966 | 20 | 12 | 2 | 1 | 21 | 23 | 19 | 25 | H1a | 221 |
| a-RM7115 | Dominican Republic | 1980s | 21 | 12 | 9 | 7 | 19 | 24 | 19 | 62 | I1b | 230 |
| a-RM6062 | England | 1965 | 21 | 13 | 9 | 7 | 19 | 24 | 19 | 20 | I1a | 227 |
| a-RM6069 | England | 1963 | 21 | 13 | 9 | 7 | 19 | 24 | 19 | 20 | I1a | 228 |
| a-RM6080 | England | 1967 | 21 | 13 | 9 | 7 | 19 | 24 | 19 | 20 | I1a | 229 |
| b-RM1324 | United States | 1947 | 21 | 12 | 9 | 4 | 5 | 20 | 18 | 61 | J3 | 239 |
| e-RM6158 | England | 1962 | 7 | 6 | 3 | 7 | 10 | 27 | 10 | 52 | F2a | 197 |
| e-RM6169 | England | 1964 | 18 | 6 | 3 | 8 | 23 | 28 | 19 | 27 | F1 | 189 |
| e-RM6181 | England | 1965 | 18 | 6 | 3 | 10 | 11 | 28 | 14 | 17 | F2b | 202 |
| e-RM6167 | England | 1963 | 18 | 6 | 3 | 7 | 25 | 28 | 12 | 67 | F2c | 204 |
| e-RM7066 | Papua New Guinea | 1980s | 18 | 6 | 3 | 7 | 24 | 28 | 19 | 28 | F2b | 203 |
| e-RM6185 | England | 1965 | 18 | 6 | 3 | 7 | 10 | 28 | 12 | 18 | F1 | 190 |
| e-RM6229 | England | 1977 | 18 | 6 | 3 | 7 | 10 | 28 | 12 | 18 | F2c | 215 |
| e-RM7280 | Malaysia | 1973 | 18 | 6 | 3 | 7 | 10 | 28 | 12 | 18 | F2c | 206 |
| e-RM6210 | England | 1972 | 18 | 6 | 26 | 7 | 10 | 28 | 12 | 68 | F2a | 196 |
| e-RM7423 | Kenya | 1986 | 19 | 6 | 3 | 10 | 10 | 28 | 14 | 32 | G1 | 218 |
| e-RM1018 | United States | Before 1983 | 18 | 6 | 3 | 11 | 10 | 28 | 12 | 66 | F2a | 191 |
| e-RM8031 | United States | 1983 | 18 | 6 | 3 | 22 | 10 | 28 | 12 | 69 | F2a | 192 |
| f-RM7290 | Malaysia | 1970s | 22 | 19 | 11 | 9 | 22 | 19 | 15 | 29 | K1c | 251 |
| f-RM7283 | Malaysia | 1970s | 22 | 18 | 11 | 11 | 22 | 19 | 15 | 26 | K1c | 249 |
| f-RM7298 | Malaysia | 1970s | 22 | 18 | 11 | 11 | 22 | 19 | 15 | 26 | K1c | 250 |
| f-RM7417 | Kenya | 1986 | 22 | 19 | 11 | 11 | 35 | 32 | 15 | 63 | L1 | 267 |
| f-RM6237 | England | 1963 | 22 | 6 | 11 | 12 | 1 | 18 | 15 | 16 | K2a | 265 |
| f-RM6244 | England | 1964 | 22 | 6 | 11 | 12 | 1 | 18 | 15 | 16 | K2a | 263 |
| f-RM6255 | England | 1967 | 22 | 6 | 11 | 12 | 1 | 18 | 15 | 16 | K2a | 257 |
| f-RM6252 | England | 1966 | 22 | 6 | 11 | 11 | 12 | 18 | 15 | 15 | K1b | 241 |
a
The prefixes a-, b-, c-, d-, e-, f- and nt- indicate the serotype of the isolate, or that it is nontypeable (noncapsulated [nt]). Isolates beginning with the prefix “b(iv)” are serotype b, biotype iv. STs are listed in their order on the minimum evolution tree (Fig. 3). Isolates of group I are separated by a blank line from those of group II.
b
The MLEE lineage and ET are shown, where known, for isolates previously characterized by MLEE (28).