TABLE 2.
Base sequences and gene locations for the seg-sej-specific 5′-biotinylated oligonucleotide probes
| Genea | Probeb | Oligonucleotide sequence (5′-3′) | Location within gene (nucleotide range) |
|---|---|---|---|
| segc | SEG-8B | GAT TCA TTA CAT TAC CCA TAG TTC CC | 555-580 |
| seh | SEH-3B | ACT GCT GAT TTA GCT CAG AAG TTT A | 454-478 |
| sei | SEI-3B | ACA CTG GTA AAG GCA AAG AAT ATG | 656-679 |
| sej | SEJ-3B | GGG TAT CTC TGA AAA GAT AAT GAC A | 1087-1111 |
Sequences and locations were derived from the published nucleotide sequences for seg (30), seh (34), sei (30), and sej (42).
5′ biotinylated.
The probe sequence used here covers also the nucleotide sequence of variant SEG (1).