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. 2000 Jan 18;97(2):704–709. doi: 10.1073/pnas.97.2.704

Figure 1.

Figure 1

Sequence similarity of Ubx-like Hox genes. (A) Alignment of regions with sequence similarity of Drosophila Ubx (GenBank accession no. P02834), onychophora Ubx (Acanthokara kaputensis, AAB92412), leech Lox2 (Helobdella robusta, A49130), leech Lox4 (Hirudo medicinalis, AAB35067), and Drosophila abd-A (P29555). Note that the amino-terminal peptide of Lox4 differs from previously published sequence (34), as independent sequencing of the Lox4 cDNA indicated an early frameshift and a different predicted protein sequence. Amino acid differences between DUbx and the other sequences are highlighted within the extended homeodomain. Asterisks mark the residues in the carboxyl-terminal tail of DUbx1a that have been mapped as the epitope of the cross-reactive monoclonal antibody FP6.87 (11). (B) Constructs used to overexpress Ubx orthologs and chimeras in Drosophila. Full-length constructs: UAS-DUbx1a (Drosophila melanogaster; ref. 31), UAS-OUbx (onychophora). Chimeric constructs: UAS-Ubx1a/OnyHD, UAS-Ubx1a/Lox2HD, UAS-Ubx1a/Lox4HD. In the chimeric constructs the extended homeodomain region of DUbx1a is replaced by the homologous sequences of OUbx, Lox2, and Lox4, respectively. The number of incorporated amino acid substitutions is indicated for each chimeric construct.