TABLE 7.

YMLDLQPET tetramer-sorted CD8⁺ T cells recognize a peptide provided from the coronavirus NS2 gene product^a

Target	Peptide	Amt of GM-CSF produced (pg/ml/48 h) by T cells from patient in indicated expt
		7		13		14
		A	B	A	B	A	B
T2 cells pulsed with:
Medium alone		0	0	0	0	0	0
HPV16 E7	YMLDLQPET	37	43	51	12	27	30
Human DNA clone 342	YMLILHPET	10	7	0	0	13	0
Coronavirus NS2	TMLDIOPED	139	50	11	5	123	NDb
gp100 (control)	YLEPGPVTA	0	0	0	0	0	0

^aYMLDLQPET tetramer-reactive T cells from PBL from patient 7 with CIN (Table 5) or patients 13 and 14 with invasive cervical cancer (Table 6) were isolated as described in detail in Materials and Methods. The patient designation corresponds to the numbering in Tables 5 and 6. Two independent experiments (this table and Table 8) have been performed. HPV16 E7-isolated T cells recognize the nominal T-cell epitope as well as the peptide derived from the coronavirus NS2 gene product. T2 cells alone, or those pulsed with a self peptide, or a non-HPV-related (melanoma-associated peptide, i.e., gp 100) peptide, were not recognized. T cells responded with GM-CSF production but not with IFN-γ production. Due to the low number of tetramer-sorted T cells, blocking experiments using the anti-MHC-I-specific MAb W6/32 could not be performed. A and B indicate two independent experiments.

^bND, not determined.