FIG. 1.

Tumor growth and infiltration in NOG mice. (A) Photographs of normal NOG mice and those inoculated with ED-40515(−) cells subcutaneously in the postauricular region after 3 weeks. H&E staining of tumor tissue of an ED-40515(−) injected mouse (B) and a section of the tumor-bearing liver of an SLB-1-inoculated mouse (C). In vivo expression of CD4 and CD25 are revealed by immunohistochemistry. Immunohistochemical staining using anti-CD4 (D) and anti-CD25 (E) was conducted on tumor tissues from mice 2 weeks after inoculation of the ED-40515(−) cell line.