FIG. 5.

Effect of Bay 11-7082 on primary and infiltrative tumor cell growth. Mice were injected subcutaneously via the postauricular region with ED-40515(−) cells (10⁷). At the same time, either 1% DMSO or Bay 11-7082 (20 mg/kg/day) was administered intraperitoneally to the mice every day for 20 days. Another group of experiments were conducted using administration of the same doses of DMSO or Bay 11-7082 at the tumor site 10 days after tumor formation for 15 days. May-Grunwald and Giemsa staining showed infiltration of tumor cells in PBMNCs of mice treated with DMSO (A) or Bay 11-7082 (B) after 3 weeks, respectively. Photographs of subcutaneously formed ED-40515(−) tumors (C) and tumor volume (D) in the mice that had received DMSO or Bay 11-7082 10 days after tumor formation at the tumor site. Black bars represent the DMSO-treated control mice, and open bars represent the Bay 11-7082-treated mice. Each result was obtained from three different mice (means are shown [error bars, standard errors]).