TABLE 2.
Results of CAT assay of transiently transfected HeLa cells with NRE deletion mutant constructs
| Construct | Position (size of deletion in genome) (both in nt) | CAT activity (cpm/103 cells; mean ± SD) | Fold increase over wild-type construct (pCATPE445) |
|---|---|---|---|
| pCATPE445 | 4.71 ± 0.56 | ||
| pCAT5′ | 7177-7206 (30) | 13.90 ± 2.48 | 3.0 |
pCAT5′
|
7191-7206 (16) | 14.48 ± 3.55 | 3.1 |
| pCAT3′ | 7129-7176 (49) | 46.54 ± 3.95 | 9.8 |
pCAT3′
|
7129-7160 (33) | 40.38 ± 3.48 | 8.6 |
| pCATSE227 | 7008-7227 (219) | 200.46 ± 14.53 | 42.5 |
| pBluescript KS(+) | 1.70 ± 0.9 |