TABLE 3.
Results of CAT assay of transiently transfected HeLa cells with splice site mutant constructs
| Construct | CAT activity (cpm/103 cells; mean ± SD) | Fold increase over wild-type construct (pCATPE445) |
|---|---|---|
| pCATPE445 | 4.45 ± 2.19 | |
| pCATwt2SD | 1.74 ± 0.75 | 0.4 |
| pCATmut1-4SD | 65.15 ± 9.70 | 14.6 |
| pCAT5′mut1-3SD | 93.84 ± 26.27 | 21 |
| pCAT5′mut1-4SD | 180.54 ± 11.58 | 40.5 |
| pCAT5′ | 21.56 ± 4.97 | 4.8 |
| pCAT5′mut1SD | 41.62 ± 8.03 | 9.3 |
| pCAT5′mut2SD | 93.13 ± 3.53 | 20.9 |
| pCAT5′mut3SD | 57.94 ± 4.49 | 13.0 |
| pCAT5′mut4SD | 44.98 ± 3.07 | 10.1 |
| pCATSE227 | 182.37 ± 11.67 | 40.9 |
| pBluescript KS(+) | 1.22 ± 0.10 |