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. 2003 May;77(9):5415–5427. doi: 10.1128/JVI.77.9.5415-5427.2003

FIG. 1.

FIG. 1.

Effect of CTIP1 and CTIP2 on HIV-1 LTR-driven transcription and on viral replication. (A) Microglial cells were cotransfected with vectors expressing HIV-1 LTR-CAT (1 μg) in the presence of increasing amounts (0.1, 0.5, and 1 μg) of CTIP1 and CTIP2, as indicated. After 2 days, CAT activities were measured and are expressed relative to the value obtained with LTR-CAT alone. Values correspond to an average of at least three independent experiments done in duplicate. Error bars, standard deviations. (B) Cells were cotransfected with HIV-1 pNL4-3 (1.5 μg) and with increasing amounts (0.1, 0.5, and 1 μg) of the indicated CTIP1 and CTIP2 expression vectors. Two days after transfection, samples of the culture supernatant were analyzed for p24 Gag content. Data represent an average of four independent experiments performed in duplicate. They are expressed relative to the value obtained with pNL4-3 alone taken as 1. Depending on the cell confluency, this value varied between 500 and 5,000 pg/ml.