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. 2003 May;77(9):5415–5427. doi: 10.1128/JVI.77.9.5415-5427.2003

FIG. 9.

FIG. 9.

Full-length and 145-434 CTIP2 inhibit HIV-1 LTR-driven transcription and viral replication. (A) Microglial cells were cotransfected with vectors expressing HIV-1 LTR-CAT (1 μg) and Tat (2 ng) in the presence of the indicated CTIP2 deletion mutants (1 μg). CAT assays were performed after 2 days. CAT activities are expressed relative to the value obtained with LTR-CAT alone. Values correspond to an average of at least three independent experiments done in duplicate. (B) Western blot analysis of nuclear proteins extracted from cells transfected for 48 h with the indicated expression vectors used in panel A. The blot was probed with anti-Flag antibodies. Positions of size standards are indicated in kilodaltons. (C) Cells were cotransfected with vectors expressing HIV-1 pNL4-3 (1.5 μg) and the indicated CTIP2 deletion mutants (1 μg). Two days after transfection, samples of the culture supernatant were analyzed for p24 Gag content. Data represent an average of at least three independent experiments performed in duplicate. They are expressed relative to the value obtained with pNL4-3 alone taken as 1, which corresponds to an average of 2000 pg/ml of p24 Gag.