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Cellular fractionation following the binding of VP-14637. Hep-2 cells were infected with RSV at an MOI of 0.1 or mock infected for 48 h, followed by incubation with 10 nM [3H]VP-14637 for 2 h. The cells were subjected to Dounce homogenization and centrifugation to yield the indicated fractions. The samples were read in a scintillation counter to determine the level of bound [3H]VP-14637 in each fraction. The numbers above the bars indicate the percentage of compound in each fraction.
