FIG. 9.

VP-14637 does not bind to cells expressing F proteins that contain resistance mutations. (A) CEF cells were infected, transfected, and analyzed as described in the legend to Fig. 6. Genes encoding wild-type F (WT), one of the two point mutations (F488Y or T400A), or an empty control plasmid were used for transfection. (B) FACS analysis was performed on the cells to determine surface expression of the F proteins. The histograms are plotted as cell count versus F expression level. The solid histograms represent the control cells, and the open histograms correspond to cells expressing the indicated F proteins.