FIG. 3.

Luciferase activities of various P/L replicons as a measure of RNA replication. (A) RNAs from dual-cre P/L replicons with second rescuer cre elements derived from poliovirus, HRV2, or HRV14. Transfection of RNA and luciferase activity measurements are described in Materials and Methods. Even-numbered lanes, transfections repeated in the presence of 2 mM guanidine-HCl (GnHCl). arts, artificial. (B) Dual-cre luciferase replicons with the rescuer cre from poliovirus carrying point mutations in G₁, A₂, and A₁₄ in the core cre sequence were tested for their ability to replicate in HeLa R19 cells. The nomenclature is as follows. P/L refers to the parental replicon plasmid followed by A₅C when it carries an inactivating mutation in the 2C^ATPase coding sequence. This is followed by the sequences of the rescuer cre and the insertion site (N, NheI; E, EcoRI; S, SacI).