FIG. 7.

In vitro uridylylation using shortened PV-cre(2C) RNAs as templates. (A) Effect of truncation on the ability of PV-cre(2C) RNA to serve as the template in VPg uridylylation. The uridylylation of VPg was measured by the standard method described in Materials and Methods. The yield of the reaction (graph) was shown by autoradiography. (B) Schematic drawing of the secondary structure of complete and truncated PV-cre RNAs utilized in this experiment. The first and last nucleotides as well as the length of each RNA template are indicated.