TABLE 1.
Oligonucleotides used for mutagenesis and cloning (involving PCR amplification)
| Oligonucleotide | Sequence (5′-3′) |
|---|---|
| PVcre (SacI) forward | GCC GAG CTC ACA ACC TAT GGG TTC GGA ACT ATT AAC AAC TAC ATA C |
| PVcre (SacI) reverse | GC GAG CTC TAC TAG CAA ACA TAC TGG |
| HRV14cre (SacI) forward | GCC GAG CTC ACA ACC TAT GGG TTC GGA GCA CTC ACT GAA GGC TTA G |
| HRV14cre (SacI) reverse | GGC GAG CTC TTT TTG TGT GTG TTT TGG |
| HRV2cre (SacI) forward | GCC GAG CTC ACA ACC TAT GGG TTC GGA GAT TTA ATC ATT TAC CGA AC |
| HRV2cre (SacI) reverse | GGC GAG CTC GGG AAT GTA ATC ATC ACC |
| HRV2cre U11C forw. (QM)a | ATT TAC CGA ACA AAC ACC GTA GGT GAT GAT TAC |
| PVcre (EcoRI) forward | CGG AAT TCA CTA TTA ACA ACT ACA TAC AG |
| PVcre (EcoRI) reverse | CGG AAT TCC CCA AAG CCG TAA GTC GTT ACT AGC AAA CAT ACT GG |
| PVcre G3A C11U forw. (QM) | CAT ACA GTT CAA GAA CAA ACA CTG TAT TGA ACC AGT ATG |
| PVcre A14C forward (QM) | CAA GAG CAA ACA CCG TCT TGA ACC AGT ATG TTT G |
| PVcre G1A forward (QM) | CTA CAT ACA GTT CAA AAG CAA ACA CCG TAT TG |
| PVcre G1C forward (QM) | CTA CAT ACA GTT CAA CAG CAA ACA CCG TAT TG |
| PVcre G1U forward (QM) | CTA CAT ACA GTT CAA TAG CAA ACA CCG TAT TG |
| PVcre A14G forward (QM) | CAA GAG CAA ACA CCG TGT TGA ACC AGT ATG TTT G |
| PVcre A14U forward (QM) | CAA GAG CAA ACA CCG TTT TGA ACC AGT ATG TTT G |
| PV/Luc+(NheI) forw. (QM) | GTT TTA TAC TCC CTT CCC GCT AGC TTA GAC GCA CAA AAC CAA G |
| PVcre (NheI) forward | CTA GCT AGC ACT ATT AAC AAC TAC |
| PVcre (NheI) reverse | CTA GCT AGC TAC TAG CAA ACA TAC |
| Artificial cre (NheI) forward | CTA GCT AGC GAG CTC GAG CAA ACA CCG TAG AGC |
| Artificial cre (NheI) reverse | GAT GCT AGC GAG CTC TAC GGT GTT TGC TCG AGC |
| Artificial cre A5C(NheI) forw. | CTA GCT AGC GAG CTC GAG CCA ACA CCG TAG AGC |
| Artificial cre A5C(NheI) rev. | GAT GCT AGC GAG CTC TAC GGT GTT GGC TCG AGC |
| PVcre (SLtm) forward (QM) | CTA TTA ACA ACT ACA TAC AAT TTA AGA GCC AAC ACC GTA TCG AAC |
a
QM, oligonucleotide used for QuikChange mutagenesis.