FIG. 4.

Reactivation of persistent chlamydial organisms. The numbers of recoverable infectious chlamydial cells (in IFU) from acute infection (Acute inf.) and persistence trials based on iron depletion by addition of DAM, penicillin G (PenG) treatment, or exposure to IFN-γ (filled columns) are compared to those from the reactivation assay, which included removal of persistence inducers at 24 h p.i. (open columns), and 48 h p.i. (shaded columns) and subsequent culture in complete growth medium for 24 h.