FIG. 2.

Neutrophils are the major source of BPI in lung fluid from CF patients. Isolated cells from sputum samples (A and B) or BAL (C) of CF patients were analyzed by flow cytometry. Cell types were distinguished by extracellular staining of CD66b for granulocytes and CD14 for monocytes. BPI was detected by intracellular staining. Percentages of the total amount of cells are given within the charts for each quadrant. The histograms to the right show BPI-positive cells (black) versus the isotype control (white).