TABLE 1.
FACS analysis of the cellular compositions of Per2−/− mouse and control mouse spleensa
| Cell type | Mean cell composition ± SD (%) in:
|
|||
|---|---|---|---|---|
| Wild-type mice
|
Per2−/− mice
|
|||
| Unchallenged | LPS challenged | Unchallenged | LPS challenged | |
| T lymphocytes | 34.6 ± 0.6 | 25.7 ± 2.5 | 29.1 ± 2.5* | 27.3 ± 0.7 |
| B lymphocytes | 53.6 ± 0.4 | 62.5 ± 1.0 | 53.1 ± 1.0 | 53.4 ± 1.3** |
| NK cells | 2.8 ± 0.6 | 1.7 ± 0.3 | 2.7 ± 0.3 | 1.8 ± 0.2 |
| NKT cells | 1.0 ± 0.2 | 1.0 ± 0.1 | 1.2 ± 0.2 | 1.3 ± 0.1 |
| Macrophages | 4.4 ± 1.2 | 6.5 ± 1.4 | 6.2 ± 1.3 | 6.7 ± 1.4 |
| Dendritic cells | 1.5 ± 0.1 | 2.8 ± 0.4 | 2.0 ± 0.3 | 2.7 ± 0.2 |
a
Mice were challenged with LPS (25 mg/kg of body weight, intraperitoneally), and their spleens were harvested 6 h after the LPS injection. Splenocytes were subjected to FACS analysis as described in Materials and Methods. Three to eight mice of each strain were examined. ANOVA was used for multigroup comparison, followed by Tukey's multiple-comparison test if ANOVA showed a significant difference. Cell subsets that showed significant differences between wild-type and Per2−/− mice are in boldface. *, P < 0.05; **, P < 0.01.