FIG. 2.

Mutation in uvrY reduces attachment to cultured chicken fibroblasts and macrophages. The monolayers of cultured cells over glass coverslips were incubated with bacteria (grown under static conditions) with a multiplicity of infection of 10. As described in Table 5, footnote c, the unattached bacteria were washed, stained with Hema-3 stain (Fischer Scientific, Middletown, VA), and visualized using a Spot RT camera attached to a Olympus BH-2 microscope with a 100× objective lens. Attachment of E. coli χ7122 (wild type [wt]), uvrY mutant, and uvrY mutant complemented with a plasmid-borne copy of the wild-type uvrY gene (p-uvrY). (A to D) Attachment to DF-1 chicken fibroblasts. Arrows indicate surface-attached bacteria. (E to H) Attachment to HD11 chicken macrophages. Panels F and H show attached bacteria, and panel G shows bacteria that are mostly engulfed (indicated by arrows).