TABLE 1.
Primers used in this study
| Primer | Sequencea |
|---|---|
| barA knockout with kanamycin cassette | |
| OSM 58 | 5′-CATCGTCGCCATTCCGATATTGTTCGCGCGATTTCG TTGCATATGAATATCCTCC-3′ |
| OSM 59 | 5′-CGACATTATCCATCTCGTCCAACAGTTCCAGCAGCTACTTGTGTAGGCTGGAGC-3′ |
| uvrY knockout with chloramphenicol cassette | |
| OSM 43 | 5′-TGGTGCCGCCAGGGATACGACGCATTCTGGAAGTTGCATATGAATATCCTCCTTAGT-3′ |
| OSM 44 | 5′-CATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGACCGTGTAGGCTGGAGCTGCTTC-3′ |
| barA amplification and cloning primers | |
| OSM221 | 5′-CCCGAATTCATAGCATACGCCAAAATGAGGACAG-3′ |
| OSM222 | 5′-CCCGATATCATAACTCGACAAGACATCCATTA-3′ |
| uvrY amplification and cloning primers | |
| OSM 64 | 5′-CCCGAATTCATAATTTCATCGTAGGGCTTACTGTGA-3′ |
| OSM 74 | 5′-CCCCTGCAGATGCACGCCTGGCTGGTTAC-3′ |
| papA amplification primers | |
| OSM 309 | 5′-ACTCTGCGGACCACTTGGGA-3′ |
| OSM 310 | 5′-CCAACTATTCCTCAGGGGCA-3′ |
| fimA amplification primers | |
| OSM 260 | 5′-ACCGTTCAGTTAGGACAGGTTCGT-3′ |
| OSM 261 | 5′-TCTGCAGAGCCAGAACGTTTGTAT-3′ |
| 16S rrnA amplification primers | |
| OSM 17 | 5′-GTGCCCAGATGGGATTAGCTAGTAG-3′ |
| OSM 81 | 5′-GTTAGCCGGTGCTTCTTCTG-3′ |
a
The underlined sequences are restriction recognition sequences used for cloning.