TABLE 1.
Bacterial strains used in this study
| Strain | Descriptiona | Reference or source |
|---|---|---|
| E. coli | ||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tcr)] | Stratagene |
| XL2-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tcr) Amy Cmr] | Stratagene |
| S17-1 | Used for inserting mating constructs into P. aeruginosa; thi pro hsdR recA RP4-2 (Tc::Mu) (Km::Tn7) | 42 |
| P. aeruginosa | ||
| PA103 | Virulent lung isolate; known T3SS effectors are ExoT and ExoU | 31 |
| ΔretS (ΔrtsM) | PA103 with in-frame deletion of aa 37 to 924 of retS (rtsM) | 29 |
| ΔretS attB::retS | retS (expressed from its own promoter) integrated at the attB site | 29 |
| ΔretS attB::retS H424Q | retS H424Q integrated at the attB site | This study |
| ΔretS attB::retS D664A D665A | retS D664A D665A integrated at the attB site | This study |
| ΔretS attB::retS D713A | retS D713A integrated at the attB site | This study |
| ΔretS attB::retS E814A D815A | retS E814A D815A integrated at the attB site | This study |
| ΔretS attB::retS D858A | retS D858A integrated at the attB site | This study |
| ΔretS attB::retS H424Q D713A | retS H424Q D713A integrated at the attB site | This study |
| ΔretS attB::retS H424Q D858A | retS H424Q D858A integrated at the attB site | This study |
| ΔretS attB::retS D713A D858A | retS D713A D858A integrated at the attB site | This study |
| ΔretS attB::retS H424Q D713A D858A | retS H424Q D713A D858A integrated at the attB site | This study |
| ΔretS attB::retS Δ37-185 | retS Δ37-185 integrated at the attB site | This study |
| ΔretS attB::retS Δ37-360 | retS Δ37-360 integrated at the attB site | This study |
a
Abbreviations: Ap, ampicillin; Cb, carbenicillin; Cm, chloramphenicol; Gm, gentamicin; Km, kanamycin; Tc, tetracycline; aa, amino acids.