TABLE 3.
Primer sequences used in this study
| Primer | Sequencea |
|---|---|
| exoT-RT-PCR-1 | 5′-CCGCCAGCCAGCATGTACTC-3′ |
| exoT-RT-PCR-2 | 5′-GGGTGTTCGCCGAGGTACTG-3′ |
| pelA-RT-PCR-1 | 5′-GGCGCTCACCGTACTCGATC-3′ |
| pelA-PCR-2 | 5′-GATGCGCCGGCCATTCTCG-3′ |
| pslD-RT-PCR-1 | 5′-CGCACGCCGCAGGAAATCG-3′ |
| pslD-RT-PCR-2 | 5′-CTTCCGAGTCCTCGCGCATG-3′ |
| rsmZ-RT-PCR-1 | 5′-CGTACAGGGAACACGCAAC-3′ |
| rsmZ-RT-PCR-2 | 5′-AAAAAGGGGCGGGGTAATAC-3′ |
| rplU-RT-PCR-1 | 5′-CGCAGTGATTGTTACCGGTG-3′ |
| rplU-RT-PCR-2 | 5′-AGGCCTGAATGCCGGTGATC-3′ |
| H424Q-1 | 5′-GCCAAGATCAGCCAGGAGATCCGCACGC CC-3′ |
| H424Q-2 | 5′-GGGCGTGCGGATCTCCTGGCTGATCTTGGC-3′ |
| D664A D665A-1 | 5′-GCTGCTGGTGGTGGCCGCCAACGAAACCTGCCGC-3′ |
| D664A D665A-2 | 5′-GCGGCAGGTGGCGTTGGCGGCCACCACCAGCAGC-3′ |
| D713A-1 | 5′-GTGGTCCTGCTCGCCCAGGACATGCCC-3′ |
| D713A-2 | 5′-GGGCATGTCCTGGGCGAGCAGGACCAC-3′ |
| E814A D815A-1 | 5′-GATCCTCGTCGCCGCGGCCAACAGCATCTCC-3′ |
| E814A D815A-2 | 5′-GGAGATGCTGTTGGCCGCGGCGACGAGGATC-3′ |
| D858A-1 | 5′-CTGGTGCTGATGCGGTGCGAGATGCCG-3′ |
| D858A-2 | 5′-CGGCATCTCGCAGGCCATCAGCACCAG-3′ |
| RetS 1-36-1 | 5′-GCGCGGCCGCGTGCTCTGTG-3′ |
| RetS 1-36-2 | 5′-GCACTAGTGGGAACGCTGGAAACGCCG-3′ |
| RetS 186-500-1 | 5′-GCACTAGTAAGCCCGCCTACGCCTTC-3′ |
| RetS 186-500-2 | 5′-GCGATATCCAGGCAGTCCTCG-3′ |
| RetS 361-500-1 | 5′-GCACTAGTCAACTCGATCCGGGCTG-3′ |
| RetS 361-500-2 | 5′-GCGATATCCAGGCAGTCCTCG-3′ |
a
Restriction enzyme sites are underlined.