TABLE 4.

Transcription of the T3S effector ExoT in strains containing point mutations in RetS

Bacterial strain	pg exoT mRNA/pg rplU mRNAa	Expression relative to PA103 (% ± SD)
PA103	0.981 ± 0.067	100 ± 6.8
ΔretS	0.017 ± 0.005	1.70 ± 0.46
ΔretS attB::retS	0.700 ± 0.135	71.4 ± 13.8
ΔretS attB::retS H424Q	0.113 ± 0.004	11.5 ± 0.4
ΔretS attB::retS D664A D665A	0.779 ± 0.116	79.4 ± 11.8
ΔretS attB::retS D713A	1.078 ± 0.131	109.9 ± 13.4
ΔretS attB::retS E814A D815A	0.033 ± 0.004	3.35 ± 0.39
ΔretS attB::retS D858A	0.074 ± 0.009	7.59 ± 0.89
ΔretS attB::retS H424Q D713A	0.267 ± 0.053	27.2 ± 5.4
ΔretS attB::retS H424Q D858A	0.055 ± 0.006	5.56 ± 0.58
ΔretS attB::retS D713A D858A	0.048 ± 0.004	4.87 ± 0.44
ΔretS attB::retS H424Q D713A D858A	0.048 ± 0.007	4.84 ± 0.69

^aThe values are expressed as the average of the ratio of pg of exoT mRNA per pg of rplU mRNA ± standard deviation (n = 3). The results are representative of three independent experiments.