TABLE 4.
Bacterial strain | pg exoT mRNA/pg rplU mRNAa | Expression relative to PA103 (% ± SD) |
---|---|---|
PA103 | 0.981 ± 0.067 | 100 ± 6.8 |
ΔretS | 0.017 ± 0.005 | 1.70 ± 0.46 |
ΔretS attB::retS | 0.700 ± 0.135 | 71.4 ± 13.8 |
ΔretS attB::retS H424Q | 0.113 ± 0.004 | 11.5 ± 0.4 |
ΔretS attB::retS D664A D665A | 0.779 ± 0.116 | 79.4 ± 11.8 |
ΔretS attB::retS D713A | 1.078 ± 0.131 | 109.9 ± 13.4 |
ΔretS attB::retS E814A D815A | 0.033 ± 0.004 | 3.35 ± 0.39 |
ΔretS attB::retS D858A | 0.074 ± 0.009 | 7.59 ± 0.89 |
ΔretS attB::retS H424Q D713A | 0.267 ± 0.053 | 27.2 ± 5.4 |
ΔretS attB::retS H424Q D858A | 0.055 ± 0.006 | 5.56 ± 0.58 |
ΔretS attB::retS D713A D858A | 0.048 ± 0.004 | 4.87 ± 0.44 |
ΔretS attB::retS H424Q D713A D858A | 0.048 ± 0.007 | 4.84 ± 0.69 |
The values are expressed as the average of the ratio of pg of exoT mRNA per pg of rplU mRNA ± standard deviation (n = 3). The results are representative of three independent experiments.