TABLE 5.

Transcription of the small regulatory RNA rsmZ in strains containing point mutations in RetS

Bacterial strain	pg rsmZ mRNA/pg rplU mRNAa	Expression relative to PA103 (% ± SD)
PA103	0.033 ± 0.009	100 ± 27
ΔretS	0.593 ± 0.077	1,810 ± 230
ΔretS attB::retS	0.090 ± 0.049	274 ± 149
ΔretS attB::retS H424Q	0.469 ± 0.082	1,430 ± 250
ΔretS attB::retS D713A	0.078 ± 0.009	237 ± 27
ΔretS attB::retS D858A	0.493 ± 0.177	1,500 ± 540
ΔretS attB::retS H424Q D713A	0.226 ± 0.049	688 ± 149
ΔretS attB::retS H424Q D858A	0.455 ± 0.164	1,380 ± 500
ΔretS attB::retS D713A D858A	0.740 ± 0.218	2,250 ± 660
ΔretS attB::retS H424Q D713A D858A	0.689 ± 0.118	2,100 ± 360

^aThe values are expressed as the average of the ratio of pg of rsmZ mRNA per pg of rplU mRNA ± standard deviation (n = 3). The results are representative of three independent experiments.