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Western blot analysis of antigen-encoding clones. All membranes were blotted with human sera, which identified the original clone to confirm its suitability for overexpression. In all blots, lane A contained a negative control (E. coli XLOLR/pBK-CMV). The proteins encoded by the clones in the blots were Atl Clone3 (blot 1), GehD (blot 2), ScaA (blot 3), and ScaB (blot 4).
