TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| S. epidermidis strains | ||
| 138 | Clinical isolate | 32 |
| Hay | ATCC 55133 | ATCC |
| E. coli strains | ||
| XL-1 Blue MRF′ | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac (F′ proAB lacIqZΔM15 Tn5 [Tcr]) | Stratagene |
| XLOLR | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA thi-1 recA1 gyrA96 relA1 lac (F′ proAB lacIqZΔM15 Tn5 [Tcr]) Su− λr | Stratagene |
| BL21(DE3) | F−ompT gal (dcm) (lon) hsdSB (rB− mB−) | Novagen |
| Plasmids | ||
| pET21-a | His6-tagged overexpression vector; Apr | Novagen |
| pET24-d | His6-tagged overexpression vector; Kmr | Novagen |
| pMA | pET24-d containing internal fragment encoding AtlE amidase domain; Kmr | This study |
| pML | pET24-d containing internal fragment encoding GehD; Kmr | This study |
| pRSN | pET21-a containing internal fragment encoding AtlE repeat sequence; Apr | This study |
| pPP | pET24-d containing internal fragment encoding AtlE propeptide; Kmr | This study |
| pScaA | pET24-d containing internal fragment encoding ScaA; Kmr | This study |
| pScaB | pET24-d containing internal fragment encoding ScaB; Kmr | This study |