TABLE 2.
Summary of six serial propagations of E. bieneusi in rodentsa
| Rodents | Passage no. | No. of animals | Inoculum (no. of spores) | No. of spores recovered fromb:
|
|
|---|---|---|---|---|---|
| Feces | Gut contents | ||||
| SCID mice | 1 | 5 | 1 × 103 | (3.2-8.8) × 104 | (0.5-2.0) × 106 |
| RNU | 2 | 4 | 1 × 104 | NDc | ND |
| 3 | 5 | 1 × 104 | (0.2-1.0) × 107 | (0.8-4.0) × 107 | |
| 4 | 4 | 1 × 104 | ND | (2.0-4.7) × 107 | |
| 5 | 5 | 1 × 104 | (2.0-4.6) × 107 | (1.8-6.3) × 107 | |
| 6 | 5 | 1 × 104 | ND | ND | |
| Control RNUd | 1 | 5 | 0 | 0 | 0 |
a
Mice were given 1 mg and rats 5 mg of anti-IFN-γ MAb intraperitoneally at challenge and 1 week later and were euthnatized on day 14.
b
Spores were recovered either from feces at 2 weeks after oral challenge or from gut contents at euthnasia 2 weeks after infection.
c
ND, not determined.
d
Gut contents of an uninfected control RNU from Table 1 were used to inoculate control RNU.