FIG. 6.

Ex vivo analysis of CD4⁺ and CD8⁺ T-cell percentages in harvested splenocytes from the intradermally and intraperitoneally infected mice on day 7 postinfection. Harvested splenocytes were incubated concurrently with fluorescein isothiocyanate-conjugated anti-CD4 and phycoerythrin-conjugated anti-CD8 antibodies. Lymphocytes were gated based on size and granularity, and 20,000 events were collected. The intradermally inoculated animals had significantly (P < 0.01) higher percentages of both CD4⁺ and CD8⁺ T cells in the spleen compared to the intraperitoneally infected mice. Results are representative of all three mice per group, and similar results were obtained from three independent experiments.