FIG. 8.

TMHMM analysis of NS4B with the prediction constrained by the glycosylation results for constructs E33 (lumenal), E92 (cytoplasmic), E135 (cytoplasmic), E161 (lumenal), and E210 (cytoplasmic). The 261 amino acid residues of NS4B are indicated on the x axis. The red line describes the probability of that part of the protein to be a transmembrane domain, the blue line describes the probability of it being inside the membrane (cytoplasmic), and the green line describes the probability of its being outside the membrane (lumenal). Our designations of the different transmembrane domains are presented above the figure (TMX and TM1 to -4).