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Analysis of selv and selu2 transcripts by RT-PCR. cDNA was prepared from S. aureus A900624 total RNA using the RT primers shown (RT), followed by PCR using primer pairs A to E (boxed). Lanes A to E, results obtained using the corresponding primer pairs. Lane 1, 1-kb molecular size marker; lane 2, RT-PCR negative control (heat inactivation of reverse transcriptase); lanes A1 to E1, RT-PCR from extract of A900624; lanes A2 to E2, PCR positive control (A900624 DNA as template).
